Research Articles
RNA Integrity Number (RIN): The Definitive Guide to Requirements for Successful RNA Sequencing
This comprehensive guide details the critical role of the RNA Integrity Number (RIN) in ensuring successful RNA sequencing (RNA-Seq) for researchers and drug development professionals.
The Critical Link: How Low RNA Yield Compromises Sequencing Library Complexity and Data Integrity
For researchers and drug development professionals, obtaining sufficient and high-quality RNA is a fundamental challenge that directly impacts the success of next-generation sequencing (NGS).
Solving the Low RNA Yield Puzzle: Causes, Troubleshooting, and Optimized Protocols for Reliable Sequencing
Inadequate RNA yield and quality are critical, yet often preventable, bottlenecks that compromise the reliability and interpretability of RNA sequencing experiments.
Cross-Platform RNA-Seq Analysis: A Comprehensive Guide for Robust Transcriptomic Profiling in Biomedical Research
This article provides a comprehensive framework for cross-platform RNA-seq comparison, addressing critical challenges and solutions for researchers and drug development professionals.
From Sequencing to Significance: A Comprehensive Guide to Experimentally Validating RNA-seq Findings
This article provides a comprehensive roadmap for researchers and drug development professionals to bridge the gap between computational RNA-seq discoveries and biologically validated results.
Ensuring Reproducibility in Bulk RNA-seq Studies: A Comprehensive Guide from Foundational Challenges to Advanced Solutions
Bulk RNA-seq is a powerful tool for transcriptome analysis, but its reproducibility is challenged by technical variability, analytical choices, and frequent underpowering in experimental design.
RNA-seq Software Showdown: A Performance Evaluation Guide for Precision Research
This article provides a comprehensive performance evaluation of RNA-seq software, tailored for researchers and drug development professionals.
Bulk vs Single-Cell RNA Sequencing: A Comprehensive Guide for Researchers
This article provides a definitive comparison of bulk and single-cell RNA sequencing for researchers and drug development professionals.
From Bulk to Spatial Context: Validating and Integrating Transcriptomics Data for Advanced Biomedical Discovery
This article provides a comprehensive framework for researchers and drug development professionals to validate and integrate bulk RNA-seq data with cutting-edge spatial transcriptomics (ST) technologies.
Salmon & Kallisto vs Alignment-Based Tools: A Researcher's Guide to RNA-Seq Quantification
This article provides a comprehensive guide for researchers and drug development professionals on selecting and applying RNA-seq quantification methods.