Research Articles
From Sequence to Structure: Advances and Challenges in Computational RNA Secondary Structure Prediction
This article provides a comprehensive overview of the evolving landscape of RNA secondary structure prediction, a critical task for understanding RNA function in health and disease.
Long Non-Coding RNAs in Human Disease: From Molecular Mechanisms to Clinical Applications
Long non-coding RNAs (lncRNAs), once considered 'genomic dark matter,' are now recognized as crucial regulators of gene expression in physiological and pathological states.
Small Nuclear RNA Glycosylation: Unveiling a New Frontier in RNA Biology and Therapeutic Development
This article explores the groundbreaking discovery of small nuclear RNA (snRNA) glycosylation, a phenomenon that bridges the fields of RNA biology and glycobiology.
Cell Surface RNA: From Foundational Biology to Therapeutic Innovation
This article synthesizes the rapidly advancing field of cell surface RNA localization, a paradigm-shifting concept where nuclear-encoded RNAs are stably displayed on the extracellular face of the plasma membrane.
A-to-I RNA Editing: From Molecular Mechanism to Therapeutic Application in Biomedicine
Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, represents the most prevalent post-transcriptional modification in humans, dynamically expanding transcriptome and proteome diversity.
RNA-Binding Proteins: Master Regulators of Gene Expression and Emerging Therapeutic Targets
This article provides a comprehensive overview of the critical roles RNA-binding proteins (RBPs) play in post-transcriptional gene regulation, a rapidly advancing field with profound implications for understanding disease and developing...
RNA Structure and Dynamics: From Molecular Foundations to Therapeutic Design
This article provides a comprehensive exploration of RNA structure and dynamics, tailored for researchers and drug development professionals.
GlycoRNA: Unveiling a New Frontier in RNA Biology and Its Biomedical Applications
This article provides a comprehensive overview of glycoRNA, a recently discovered class of small non-coding RNAs modified with glycans.
A Comprehensive Guide to Single-Cell RNA Sequencing Protocols: From Experimental Design to Data Validation
This article provides a complete roadmap for researchers and drug development professionals designing single-cell RNA sequencing (scRNA-seq) experiments. It covers foundational principles, from understanding cellular heterogeneity and the critical importance of Unique Molecular Identifiers (UMIs) to selecting the appropriate isolation method for your sample type. The guide details step-by-step methodological workflows for both high- and low-throughput platforms, offers solutions for common pitfalls in tissue dissociation and batch effects, and outlines essential techniques for validating scRNA-seq findings through spatial transcriptomics, protein-level assays, and functional studies. By integrating established best practices with the latest advancements, this resource aims to empower scientists to generate robust, reproducible, and biologically insightful single-cell data.
The Hidden Players in Leukemia: How BCR-ABL Splice Variants Challenge Targeted Therapy
Exploring how BCR-ABL splice variants in chronic myeloid leukemia create treatment resistance and challenge targeted therapy approaches.