Research Articles
Bulk RNA Sequencing for Fusion Gene Detection: A Comprehensive Guide for Researchers and Clinicians
Fusion genes are critical drivers in cancer and other diseases, serving as vital diagnostic biomarkers and therapeutic targets.
A Comprehensive Guide to GSEA and Pathway Analysis for Bulk RNA-seq Data
This article provides a complete resource for researchers and bioinformaticians applying Gene Set Enrichment Analysis (GSEA) to bulk RNA-seq data.
eQTL Mapping: From Foundational Concepts to Advanced Applications in Drug Discovery
This comprehensive review explores expression quantitative trait loci (eQTL) mapping as a pivotal methodology bridging genetic variation and gene expression.
Bulk RNA-seq Normalization Methods Decoded: A Practical Guide to TMM, RPKM, FPKM, and Beyond for Reliable Gene Expression Analysis
This article provides a comprehensive guide for researchers and drug development professionals on navigating the critical step of normalization in bulk RNA-seq data analysis.
A Comprehensive Guide to Alternative Splicing Analysis with Bulk RNA-Seq: From Foundational Concepts to Clinical Applications
This article provides a complete roadmap for researchers and drug development professionals conducting alternative splicing (AS) analysis using bulk RNA sequencing.
RNA-seq Alignment Tools Compared: A Practical Guide to STAR, HISAT2, and TopHat2 for Biomedical Research
This article provides a comprehensive guide for researchers and drug development professionals on the critical step of read alignment in RNA-seq analysis.
A Comprehensive Guide to Bulk RNA-seq Analysis: Mastering DESeq2 and edgeR for Robust Differential Expression
This article provides a definitive guide for researchers and scientists performing differential expression analysis with DESeq2 and edgeR.
Bulk RNA-Seq Differential Expression Analysis: A Comprehensive Guide from Fundamentals to Clinical Application
This article provides a comprehensive guide to bulk RNA-seq differential gene expression (DGE) analysis, tailored for researchers, scientists, and drug development professionals.
PolyA Selection vs. Ribosomal Depletion: The Ultimate Guide to Bulk RNA-Seq Methods
This article provides a comprehensive guide for researchers and drug development professionals on choosing between polyA selection and ribosomal depletion for bulk RNA-Seq.
RNA Integrity Number (RIN) in Bulk RNA-seq: A Complete Guide from QC Fundamentals to Advanced Clinical Validation
This article provides a comprehensive resource for researchers and drug development professionals on the critical role of the RNA Integrity Number (RIN) in bulk RNA-seq workflows.