Research Articles
攻克低RNA输入的挑战：核糖体去除方法综合比较与优化指南
在转录组学研究中，对低起始量或降解的RNA样本进行有效测序面临巨大挑战，其中高达90%的核糖体RNA（rRNA）会占据宝贵的测序通量[citation:3]。本文针对研究人员、科学家和药物开发专业人员的需求，全面探讨并比较了适用于低RNA输入样本的核糖体去除策略。文章首先阐述了rRNA去除的基本原理及其在处理低质量样本（如FFPE组织、单细胞或微量活检样本）中的必要性[citation:6]。进而系统分析了基于杂交捕获、RNase H酶切及CRISPR-Cas9（如DASH）等不同方法的技术细节、商用试剂盒表现及定制化方案[citation:2][citation:4][citation:9]。文章深入探讨了针对低输入样本的实验流程优化、常见问题排查以及去除效率的验证指标。最后，通过对不同方法在灵敏度、偏倚、转录本覆盖度和成本效益方面的综合比较，为研究者根据具体样本类型和科学目标选择最优方案提供决策框架，并展望了该技术对未来生物医学与临床研究（如精准医疗和宿主-微生物组互作研究）的深远影响[citation:5][citation:7]。.
Mastering RNA Extraction: A Strategic Guide for Tissue-Specific Efficiency and Quality
For researchers in drug discovery and biomedical sciences, obtaining high-quality RNA is the critical first step for reliable gene expression analysis, RNA sequencing (RNA-Seq), and biomarker identification.
From Picograms to Precision: A Complete Guide to Validating Your Low-Input RNA Sequencing Data
This article provides a comprehensive guide for researchers and drug development professionals on validating RNA sequencing (RNA-seq) results from low-input samples.
RNA Extraction Kit Showdown: Maximizing Yield and Integrity from Low-Input Samples
For researchers working with scarce biological materials—from single cells and rare cell populations to low-biomass microbial or clinical samples—selecting the optimal RNA extraction method is critical.
Navigating Batch Effects in Low-Yield RNA-seq: A Practical Guide for Robust Transcriptomic Analysis
Batch effects present a formidable challenge in low-yield RNA-seq data analysis, where limited starting material amplifies technical variation and threatens data reliability.
Strategies for Overcoming PCR Amplification Bias in Low-Input RNA-Seq Libraries: A Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on understanding, mitigating, and validating solutions for PCR amplification bias in low RNA sequencing libraries.
Unlocking Biobank Potential: A Practical Guide to Maximizing RNA Yield and Integrity from Frozen Archived Samples
Obtaining high-quality RNA from frozen archival tissues and blood samples remains a significant bottleneck in biomedical research and drug development.
Mastering RNA Extraction: A Comprehensive Troubleshooting Guide for High-Quality Sequencing Results
This guide provides researchers and drug development scientists with a systematic framework for overcoming the critical challenges of RNA extraction in preparation for next-generation sequencing.
A Complete Guide to Detecting, Removing, and Validating Genomic DNA Contamination in RNA Samples
This article provides researchers and drug development professionals with a comprehensive resource on genomic DNA (gDNA) contamination in RNA samples—a pervasive issue that compromises data integrity in gene expression analyses.
Overcoming Incomplete RNA Solubilization: A Researcher's Guide to Reliable Extraction and Analysis
This comprehensive article addresses the critical challenge of incomplete RNA solubilization during extraction, a key bottleneck in molecular biology and therapeutic development.