Unlock the Secrets of RNA

This article provides a comprehensive comparison between strand-specific and non-strand-specific RNA sequencing for researchers and drug development professionals.

Batch effects represent one of the most significant technical challenges in RNA sequencing data analysis, capable of obscuring biological signals and leading to false discoveries.

This article provides a comprehensive guide for researchers and drug development professionals on overcoming the critical challenge of RNA degradation in sample preparation.

Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, represents the most prevalent post-transcriptional modification in humans, dynamically expanding transcriptome and proteome diversity.

This article provides a systematic evaluation of differential gene expression (DGE) analysis tools for RNA sequencing data, addressing critical considerations for researchers and drug development professionals.

This comprehensive guide explores RNA-seq differential gene expression analysis, covering foundational principles, methodological approaches, troubleshooting strategies, and validation techniques.

This article provides a comprehensive analysis of current RNA detection platforms, evaluating their technical principles, diagnostic applications, and clinical performance.

This article explores the groundbreaking discovery of GlycoRNA, a novel class of sialylated small non-coding RNAs present on the mammalian cell surface.

This article provides a comprehensive overview of mass spectrometry (MS) methodologies for the validation of RNA editing events, a critical need in epitranscriptomics research.

This article provides researchers, scientists, and drug development professionals with a complete framework for assessing RNA quality and integrity, a critical prerequisite for obtaining meaningful gene expression data from techniques...

This comprehensive guide explores Cross-Linking and Immunoprecipitation sequencing (CLIP-Seq), a transformative method for transcriptome-wide mapping of RNA-protein interactions.

This article provides a complete roadmap for researchers and drug development professionals designing single-cell RNA sequencing (scRNA-seq) experiments. It covers foundational principles, from understanding cellular heterogeneity and the critical importance of Unique Molecular Identifiers (UMIs) to selecting the appropriate isolation method for your sample type. The guide details step-by-step methodological workflows for both high- and low-throughput platforms, offers solutions for common pitfalls in tissue dissociation and batch effects, and outlines essential techniques for validating scRNA-seq findings through spatial transcriptomics, protein-level assays, and functional studies. By integrating established best practices with the latest advancements, this resource aims to empower scientists to generate robust, reproducible, and biologically insightful single-cell data.